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chip lysis buffer  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd chip lysis buffer
    Chip Lysis Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+lysis+buffer/Triton+X-100/pmc12781880-118-22-55
    Average 97 stars, based on 1403 article reviews
    chip lysis buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Replication dynamics of recombination-dependent replication forks
    Article Snippet: .. Cell pellets were resuspended in 400 μl ChIP lysis buffer (50 mM HEPES pH 7.4; 140 mM NaCl; 1% Triton X100; 0.1% NaDeoxycholate; protease inhibitors), disrupted by glass bead beating using a FastPrep-24 (MPbiomedical) homogenizer. ..

    Article Title: H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.
    Article Snippet: Received for publication, December 18, 2023, and in revised form, April 19, 2024 Published, Papers in Press, May 7, 2024, https://doi.org/10.1016/j.jbc.2024.107345 Bingxin Qin, Guangchun Lu, Xuejin Chen, Chenhua Zheng, Huanteng Lin, Qi Liu, Jinjie Shang, and Gang Feng* From the Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, China; School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China

    Article Title: H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe
    Article Snippet: For the ChIPs of Brl1-5FLAG and Ubp8-13Myc, 100 OD 600 cells were shifted to 18 °C for 6 h prior to crosslinking with 3% paraformaldehyde for 60 min, followed by fixing with 10 mM dimethyl adipimidate (Sigma, 285625) at 25 °C for 45 min. .. Cells were resuspended in the ChIP lysis buffer (50 mM HEPES (pH 7.2); 150 mM NaCl; 1 mM EDTA; 1% TritonX-100; 0.1% SDC) with yeast protease inhibitors (Sangon, C510026) and disrupted with FastPrep (MP Biomedicals) bead-beating. ..

    Article Title: A mechanism for telomere-specific telomere length regulation
    Article Snippet: .. Cells were cross-linked with 1% formaldehyde for 10 min. After stopping the cross-link reaction and washes, cells were lysed in 500 μL ChIP lysis buffer (50 mM HEPES–KOH pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8.0, 1% Triton X-100, 0.1% Na-deoxycholate, 1 mM PMSF) with glass beads using a FastPrep (MP Biomedicals). .. Following sonication, 500 μL of whole cell extract (WCE) was incubated overnight at 4°C with 2 μg anti- c -myc antibodies (mouse monoclonal, clone 9E10, Roche) for immunoprecipitation of myc-tagged proteins.

    Article Title: A mechanism for telomere-specific telomere length regulation
    Article Snippet: .. Cells were cross-linked with 1% formaldehyde for 10 min. After stopping the cross-link reaction and washes, cells were lysed in 500 μl ChIP lysis buffer (50 mM HEPES–KOH, pH 7.5, 140 mM NaCl, 1 mM ethylenediaminetetraacetic acid, pH 8.0, 1% Triton X-100, 0.1% Na-deoxycholate, 1 mM PMSF (Phenylmethanesulfonyl Fluoride)) with glass beads using a FastPrep (MP Biomedicals). .. Following sonication, 500 μl of whole cell extract was incubated overnight at 4°C with 2 μg anti- c -myc antibodies (mouse monoclonal, clone 9E10, Roche) for immunoprecipitation of myc-tagged proteins.

    Article Title: Replication dynamics of recombination-dependent replication forks.
    Article Snippet: .. Cell pellets were resuspended in 400 μl ChIP lysis buffer (50mM HEPES pH 7.4; 140mM NaCl; 1% Triton X100; 0.1% NaDeoxycholate; protease inhibitors), disrupted by glass bead beating using a FastPrep-24 (MPbiomedical) homogenizer. ..

    Lysis:

    Article Title: Replication dynamics of recombination-dependent replication forks
    Article Snippet: .. Cell pellets were resuspended in 400 μl ChIP lysis buffer (50 mM HEPES pH 7.4; 140 mM NaCl; 1% Triton X100; 0.1% NaDeoxycholate; protease inhibitors), disrupted by glass bead beating using a FastPrep-24 (MPbiomedical) homogenizer. ..

    Article Title: The SAGA histone acetyltransferase module targets SMC5/6 to specific genes
    Article Snippet: .. The yeast cell wall breakage was performed in 400 μl CHIP lysis buffer with half the volume of glass beads in 2 ml low-binding tubes using FASTprep-24. .. The suspension was washed two times with CHIP lysis buffer (15 min, 4 °C, 15,000 rpm), and 300 μl of the extract was sonicated with Bioruptor (Diagenode, 30 s ON/30 s OFF, High Power, 25 times) and clarified by centrifugation (15 min, 4 °C, 15,000 rpm), resulting in an average DNA fragment size of 300–500 bp.

    Article Title: H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe.
    Article Snippet: Received for publication, December 18, 2023, and in revised form, April 19, 2024 Published, Papers in Press, May 7, 2024, https://doi.org/10.1016/j.jbc.2024.107345 Bingxin Qin, Guangchun Lu, Xuejin Chen, Chenhua Zheng, Huanteng Lin, Qi Liu, Jinjie Shang, and Gang Feng* From the Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, China; School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China

    Article Title: SAGA histone acetyltransferase module facilitates chromatin accessibility to SMC5/6
    Article Snippet: .. The yeast cell wall breakage was performed in 400 μl CHIP lysis buffer with half the volume of glass beads in 2 ml low binding tubes using FASTprep-24. .. The suspension was washed two times with CHIP lysis buffer (15 min, 4°C, 15000 rpm), and 300 μl of the extract was sonicated with Bioruptor (Diagenode, 30 sec ON/30 sec OFF, High Power, 25 times) and clarified by centrifugation (15 min, 4°C, 15000 rpm), resulting in an average DNA fragment size of 300-500 bp.

    Article Title: H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe
    Article Snippet: For the ChIPs of Brl1-5FLAG and Ubp8-13Myc, 100 OD 600 cells were shifted to 18 °C for 6 h prior to crosslinking with 3% paraformaldehyde for 60 min, followed by fixing with 10 mM dimethyl adipimidate (Sigma, 285625) at 25 °C for 45 min. .. Cells were resuspended in the ChIP lysis buffer (50 mM HEPES (pH 7.2); 150 mM NaCl; 1 mM EDTA; 1% TritonX-100; 0.1% SDC) with yeast protease inhibitors (Sangon, C510026) and disrupted with FastPrep (MP Biomedicals) bead-beating. ..

    Article Title: A mechanism for telomere-specific telomere length regulation
    Article Snippet: .. Cells were cross-linked with 1% formaldehyde for 10 min. After stopping the cross-link reaction and washes, cells were lysed in 500 μL ChIP lysis buffer (50 mM HEPES–KOH pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8.0, 1% Triton X-100, 0.1% Na-deoxycholate, 1 mM PMSF) with glass beads using a FastPrep (MP Biomedicals). .. Following sonication, 500 μL of whole cell extract (WCE) was incubated overnight at 4°C with 2 μg anti- c -myc antibodies (mouse monoclonal, clone 9E10, Roche) for immunoprecipitation of myc-tagged proteins.

    Article Title: A mechanism for telomere-specific telomere length regulation
    Article Snippet: .. Cells were cross-linked with 1% formaldehyde for 10 min. After stopping the cross-link reaction and washes, cells were lysed in 500 μl ChIP lysis buffer (50 mM HEPES–KOH, pH 7.5, 140 mM NaCl, 1 mM ethylenediaminetetraacetic acid, pH 8.0, 1% Triton X-100, 0.1% Na-deoxycholate, 1 mM PMSF (Phenylmethanesulfonyl Fluoride)) with glass beads using a FastPrep (MP Biomedicals). .. Following sonication, 500 μl of whole cell extract was incubated overnight at 4°C with 2 μg anti- c -myc antibodies (mouse monoclonal, clone 9E10, Roche) for immunoprecipitation of myc-tagged proteins.

    Article Title: Replication dynamics of recombination-dependent replication forks.
    Article Snippet: .. Cell pellets were resuspended in 400 μl ChIP lysis buffer (50mM HEPES pH 7.4; 140mM NaCl; 1% Triton X100; 0.1% NaDeoxycholate; protease inhibitors), disrupted by glass bead beating using a FastPrep-24 (MPbiomedical) homogenizer. ..

    Binding Assay:

    Article Title: SAGA histone acetyltransferase module facilitates chromatin accessibility to SMC5/6
    Article Snippet: .. The yeast cell wall breakage was performed in 400 μl CHIP lysis buffer with half the volume of glass beads in 2 ml low binding tubes using FASTprep-24. .. The suspension was washed two times with CHIP lysis buffer (15 min, 4°C, 15000 rpm), and 300 μl of the extract was sonicated with Bioruptor (Diagenode, 30 sec ON/30 sec OFF, High Power, 25 times) and clarified by centrifugation (15 min, 4°C, 15000 rpm), resulting in an average DNA fragment size of 300-500 bp.



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